PRMT6 associates with LEF1 to enhance LEF1/beta-catenin transcriptional activity

• Main Authors: Chi-Yan Hsiao, 蕭積晏
• Other Authors: 林陽生
• Format: Others
• Language: en_US
• Published: 2004
• Online Access: http://ndltd.ncl.edu.tw/handle/97839407792241116322

碩士 === 國立臺灣大學 === 分子醫學研究所 === 93 === Wnt signals control developmental processes and its deregulation leads to various cancers. Wnt molecules transduce signals through beta-catenin/LEFs(LEF1, TCF4 and its homologs). Upon wnt signaling, beta-catenin accumulates and associates with LEFs to transactivate specific genes. LEFs are required for body plan formation, cell fate determination, cells proliferation and survival. It was well known that various proteins, such as CBP, HDAC, PIAS, and Groucho-related proteins, participated in LEFs/beta-catenin transcriptional activity. Here, we performed yeast two-hybrid screening through fetal brain libraray and mammary gland library to search additional proteins involved in LEFs/beta-catenin transcriptional activity. PRMT6 was identified to interact with LEF1 both in vivo and in vitro. It was also shown to enhance LEF1/beta-catenin transcriptional activity on reporter assay.