| Summary: | 碩士 === 國立臺灣大學 === 獸醫學研究所 === 93 === In the previous study goose parvovirus (GPV) was regarded to infect goose and Muscovy duck, and the Muscovy duck parvovirus (MDPV) was considered to infect goose only but not Muscovy duck. In our lab, a MDPV strain was isolated from goose. To understand the host range of the MDPV and its distribution in the host, white Roman goose and Muscovy duck were artificial infected with MDPV in this study.
After the inoculation of MDPV in white Roman goose and Muscovy duck, the MDPV DNA was mainly detected in liver, small intestinal and kidney. The MDPV DNA was detected in most organs at 14th day post-inoculation (DPI). The MDPV DNA continued to be detected till the end of the study at the 16th and 18th DPI. MDPV DNA could be detected in the Muscovy duck, Pekin duck, tsaiya duck , and mule duck after experimental inoculation. The result showed MDPV could infect Muscovy duck, tsaiya duck, mule duck and Pekn duck and resulting in high mortality.
To understand the occurrence of MDPV and GPV in the field, cohort study was performed in the duck farms in Yun-Lin. The results show that the vertical transmission of GPV or MDPV occurred in the field, and the occurrence of GPV might be more common than MDPV.
The nucleotide sequence of the structure protein, VP3, of the field strains of MDPV and GPV were analyzed. The results that the similarity of GPV Taiwan strain and the four isolated strains was between 97.1-99.8％. The similarity of MDPV Hungary strain and the four isolated strains was between 88.5-99.0％. The variability was higher in MDPV group than in GPV group.
|
|---|
