Whitening Activity of Chinese Herbal MedicinesThe inhibition of melanin biosynthesis of Galla Rhois on B16 cell
碩士 === 臺北醫學大學 === 醫學研究所 === 93 === Nowadays herbal medicines of whitening cosmetic are popular for women. For this reason, we attempted to find the whiting activity compound present in many herbal medicines used. First screen 70 % acetone or 50 % ethanol extracts of 30 differents kinds of herbal med...
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ndltd-TW-093TMC005340172015-10-13T11:39:21Z http://ndltd.ncl.edu.tw/handle/32758506389082946650 Whitening Activity of Chinese Herbal MedicinesThe inhibition of melanin biosynthesis of Galla Rhois on B16 cell 中草藥美白活性研究五倍子對B16黑色素細胞黑色素形成之抑制研究 Wei-Lin Chang 張瑋玲 碩士 臺北醫學大學 醫學研究所 93 Nowadays herbal medicines of whitening cosmetic are popular for women. For this reason, we attempted to find the whiting activity compound present in many herbal medicines used. First screen 70 % acetone or 50 % ethanol extracts of 30 differents kinds of herbal medicines that effects of antioxidation and melanin biosynthesis. Results showed that: Rhus javanica Linne 、Rheum palmatum Linne、Archidendron lucidum Benth、Punica granatum Linne and Rosa rugosa Thunberg showed a potent tyrosinase activity inhibition , IC50 value was 22.00, 77.75, 80.04, 120.13 and 127.00 mg/ml. The antioxidation of DPPH, IC50 value was 3.78、23.19、24.87、11.77 and 14.62 mg/ml, Rhus javanica Linne showed the most potent effect. In the cell culture assay use a-MSH、UVA induce melanogenesis in B-16 mouse melanoma cell. The results of the inhibitory effect of Rhus javanica Linne with no inducer and with inducer a-MSH co-culture and radiation UVA 2 days on melanin biosynthesis was observed in B-16 mouse melanoma cell all in a dose dependence. Then use Folin-Ciocalteu reaction and HPLC assay was used to identify the major compound: Rhus javanica Linne was richly in polyphenol and the major component was 1,2,3,4,6-Penta-O- galloyl-b-D-glucose (PG), and use the same tyrosinase activity inhibition assay PG whose IC50 value was 15 mM. By mushroom tyrosinase inhibition kinetics assay, the inhibition mechanism obtained from Lineweaver-Burk plots showed that 1,2,3,4,6-Penta-O-galloyl-b-D- -glucose is a non-competitive inhibitor. Then skin stimulate tests in animal modesl, results showing that both 2 g/g (w/w) and 1 g/g (w/w) Rhus javanica Linne, was not induce an allergic reaction in mice skin. Overall, the results indicate that the Rhus javanica Linne could be development as whitening cosmetic for skin care. Chwen-Ming Shih Ching-Chiung Wang 施純明 王靜瓊 2005 學位論文 ; thesis 93 zh-TW |
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碩士 === 臺北醫學大學 === 醫學研究所 === 93 === Nowadays herbal medicines of whitening cosmetic are popular for women. For this reason, we attempted to find the whiting activity compound present in many herbal medicines used. First screen 70 % acetone or 50 % ethanol extracts of 30 differents kinds of herbal medicines that effects of antioxidation and melanin biosynthesis. Results showed that: Rhus javanica Linne 、Rheum palmatum Linne、Archidendron lucidum Benth、Punica granatum Linne and Rosa rugosa Thunberg showed a potent tyrosinase activity inhibition , IC50 value was 22.00, 77.75, 80.04, 120.13 and 127.00 mg/ml. The antioxidation of DPPH, IC50 value was 3.78、23.19、24.87、11.77 and 14.62 mg/ml, Rhus javanica Linne showed the most potent effect. In the cell culture assay use a-MSH、UVA induce melanogenesis in B-16 mouse melanoma cell. The results of the inhibitory effect of Rhus javanica Linne with no inducer and with inducer a-MSH co-culture and radiation UVA 2 days on melanin biosynthesis was observed in B-16 mouse melanoma cell all in a dose dependence. Then use Folin-Ciocalteu reaction and HPLC assay was used to identify the major compound: Rhus javanica Linne was richly in polyphenol and the major component was 1,2,3,4,6-Penta-O- galloyl-b-D-glucose (PG), and use the same tyrosinase activity inhibition assay PG whose IC50 value was 15 mM. By mushroom tyrosinase inhibition kinetics assay, the inhibition mechanism obtained from Lineweaver-Burk plots showed that 1,2,3,4,6-Penta-O-galloyl-b-D- -glucose is a non-competitive inhibitor. Then skin stimulate tests in animal modesl, results showing that both 2 g/g (w/w) and 1 g/g (w/w) Rhus javanica Linne, was not induce an allergic reaction in mice skin. Overall, the results indicate that the Rhus javanica Linne could be development as whitening cosmetic for skin care.
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author2 |
Chwen-Ming Shih |
author_facet |
Chwen-Ming Shih Wei-Lin Chang 張瑋玲 |
author |
Wei-Lin Chang 張瑋玲 |
spellingShingle |
Wei-Lin Chang 張瑋玲 Whitening Activity of Chinese Herbal MedicinesThe inhibition of melanin biosynthesis of Galla Rhois on B16 cell |
author_sort |
Wei-Lin Chang |
title |
Whitening Activity of Chinese Herbal MedicinesThe inhibition of melanin biosynthesis of Galla Rhois on B16 cell |
title_short |
Whitening Activity of Chinese Herbal MedicinesThe inhibition of melanin biosynthesis of Galla Rhois on B16 cell |
title_full |
Whitening Activity of Chinese Herbal MedicinesThe inhibition of melanin biosynthesis of Galla Rhois on B16 cell |
title_fullStr |
Whitening Activity of Chinese Herbal MedicinesThe inhibition of melanin biosynthesis of Galla Rhois on B16 cell |
title_full_unstemmed |
Whitening Activity of Chinese Herbal MedicinesThe inhibition of melanin biosynthesis of Galla Rhois on B16 cell |
title_sort |
whitening activity of chinese herbal medicinesthe inhibition of melanin biosynthesis of galla rhois on b16 cell |
publishDate |
2005 |
url |
http://ndltd.ncl.edu.tw/handle/32758506389082946650 |
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