Whitening Activity of Chinese Herbal MedicinesThe inhibition of melanin biosynthesis of Galla Rhois on B16 cell

• Main Authors: Wei-Lin Chang, 張瑋玲
• Other Authors: Chwen-Ming Shih
• Format: Others
• Language: zh-TW
• Published: 2005
• Online Access: http://ndltd.ncl.edu.tw/handle/32758506389082946650

碩士 === 臺北醫學大學 === 醫學研究所 === 93 === Nowadays herbal medicines of whitening cosmetic are popular for women. For this reason, we attempted to find the whiting activity compound present in many herbal medicines used. First screen 70 % acetone or 50 % ethanol extracts of 30 differents kinds of herbal medicines that effects of antioxidation and melanin biosynthesis. Results showed that: Rhus javanica Linne 、Rheum palmatum Linne、Archidendron lucidum Benth、Punica granatum Linne and Rosa rugosa Thunberg showed a potent tyrosinase activity inhibition , IC50 value was 22.00, 77.75, 80.04, 120.13 and 127.00 mg/ml. The antioxidation of DPPH, IC50 value was 3.78、23.19、24.87、11.77 and 14.62 mg/ml, Rhus javanica Linne showed the most potent effect. In the cell culture assay use a-MSH、UVA induce melanogenesis in B-16 mouse melanoma cell. The results of the inhibitory effect of Rhus javanica Linne with no inducer and with inducer a-MSH co-culture and radiation UVA 2 days on melanin biosynthesis was observed in B-16 mouse melanoma cell all in a dose dependence. Then use Folin-Ciocalteu reaction and HPLC assay was used to identify the major compound: Rhus javanica Linne was richly in polyphenol and the major component was 1,2,3,4,6-Penta-O- galloyl-b-D-glucose (PG), and use the same tyrosinase activity inhibition assay PG whose IC50 value was 15 mM. By mushroom tyrosinase inhibition kinetics assay, the inhibition mechanism obtained from Lineweaver-Burk plots showed that 1,2,3,4,6-Penta-O-galloyl-b-D- -glucose is a non-competitive inhibitor. Then skin stimulate tests in animal modesl, results showing that both 2 g/g (w/w) and 1 g/g (w/w) Rhus javanica Linne, was not induce an allergic reaction in mice skin. Overall, the results indicate that the Rhus javanica Linne could be development as whitening cosmetic for skin care.