| Summary: | 碩士 === 臺北醫學大學 === 藥學系 === 93 === 英文摘要
In the course of our preliminary anticancer screeing program, we found that the MeOH extract of Ceratopteris thalictroides (L.) Brongn and Hypolepis punctata (Thumb.) Mett. exhibited cytotoxic activity. Thus. It intrigued us to further chemical investigation of these two plants. The MeOH exctract of whole fresh ferns of C. thalictroides was divided into fractions soluble in hexane, ethyl acetate, and H2O. From EtOAc fraction 22 compounds were isolated by Diaion, Sephadex LH-20, ODS, and silica gel column chromatography. Based on the 1D, 2D NMR and HRMS spectroscopic techniques, the structures were characterized as : quercetin 3-O-β-D-glucopyranoside (CT-1)、ferulic acid (CT-2)、p-coumaric acid (CT-3)、3-(4-hydroxyphenyl)-acrylic acid methyl ester (CT-4)、p-hydroxy benzaldehyde (CT-5)、β-sitosterol and β-stigmasterol mixture (CT-6)、stigmast-5-ene-3,7-dione (CT-7)、pterosin Z (CT-8)、pterosin A (CT-9)、pterosin V (CT-10)、pterosin K (CT-11)、pteroside Z (CT-12)、pterosin D 3-O-β-D-glucopyranoside (CT-13)、12-chloro-pterosin D 3-O-β-D-glucopyranoside (CT-14)、ceratopteroside B (38 mg) (CT-15)、ceratopteroside C (CT-16)、ceratopteroside D (CT-17)、ceratopteroside E (CT-18)、6-O-p-coumaroyl-D-glucopyranoside (CT-19)、3-O-p-coumaroyl
-D-glucopyranoside (CT-20)、4-O-p-coumaroyl-D-glucopyranoside (CT-21)、kaempherol 3-O-β-glucopyranoside (CT-22).Among these, compounds CT-14, CT-17, and CT-18 are newly discovered. The others, CT-1~CT-7, CT-14, CT-17~CT-18, CT-20~CT-22 were isolated from C. thalictroides (L.) Brongn. for the first time.
Similarly, the MeOH extract of fresh plant of Hypolepis punctata was divided into fractions soluble in n-hexane, ethyl acetate and H2O. These fractions were subjected to various chromatography and cytotoxic screening. Totally, six compounds were isolated from this fern, and their structures were determined to be caffeic acid (HP-1)、2R,3R pterosin-L 13-O-β-D-glucopyranoside (HP-2)、7’,8’-Dihydro-7’-(4’-hydroxy-3’-methoxyphenyl)-3- (hydroxymethyl)-1 Benzofuranpropanol 4’-O-β-glucopyranoside (HP-3)、6, 7’-dihydroxyisolariciresinol 4’-O-β-glucopyranoside (HP-4)、(+)-Lariciresinol 4’-O-β-D-glucopyranoside (HP-5)、(+)-Pinoresinol 4’-O-β-D-glucopyranoside (HP-6)。Compound HP-4 is newly discovered, and the rest five compounds were first found in this fern.
|
|---|
