Production of cyclodextrin using cyclodextrin glycosyltransferase and different starch
碩士 === 大同大學 === 生物工程學系(所) === 93 === Cyclodextrins (CDs) are cyclic oligosaccharides composed of 6-8 glucose with α-1, 4-linkages. CDs have been used in food, pharmaceutics, cosmetics industries. CDs are produced by enzymatic catalyzation of cyclodextrin glucanotransferase (CGTase) from starch. In t...
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ndltd-TW-093TTU001060072016-06-08T04:13:35Z http://ndltd.ncl.edu.tw/handle/28752290688455045548 Production of cyclodextrin using cyclodextrin glycosyltransferase and different starch 利用不同澱粉與環狀糊精葡萄糖苷轉移酶生產生產環狀糊精之研究 Gen-How Chen 陳鈞浩 碩士 大同大學 生物工程學系(所) 93 Cyclodextrins (CDs) are cyclic oligosaccharides composed of 6-8 glucose with α-1, 4-linkages. CDs have been used in food, pharmaceutics, cosmetics industries. CDs are produced by enzymatic catalyzation of cyclodextrin glucanotransferase (CGTase) from starch. In this study, CGTase is produced using starch or starch hydrolyzates as carbon sources from a Bacillus sp. BCRC 10094. The maximum CGTase activity was about 1400 unit/ml in a shaking culture using 2%(v/w)corn starch, cassava starch, soluble starch (Kanto Chemical Co. Ltd) at 33℃and 160 rpm. The carbon sources using DE10, DE20 soluble starch (Taiwan Fructose Co.,Ltd) and maltose in the medium produced less CGTase activity. The CGTase of this study and commercial CGTase (NOVO) were employed to produce CDs from 15 % (w/v) starch. CDs were produced from corn starch catalyzed by CGTase (NOVO, 250 unit/g-starch) at 75 C for 24 hrs. The yield of CDs from starch was 33 %. However, it was 1000 ~ 2000 unit needed per gram of starch for the CGTase cultured from Bacillus sp. BCRC 10094 in this study. The yield of CDs from starch was 18 ~23 % using our enzyme. The results indicated that CGTase from NOVO had better thermal stability. Kow-Jen Duan 段國仁 2005 學位論文 ; thesis 60 en_US |
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碩士 === 大同大學 === 生物工程學系(所) === 93 === Cyclodextrins (CDs) are cyclic oligosaccharides composed of 6-8 glucose with α-1, 4-linkages. CDs have been used in food, pharmaceutics, cosmetics industries. CDs are produced by enzymatic catalyzation of cyclodextrin glucanotransferase (CGTase) from starch. In this study, CGTase is produced using starch or starch hydrolyzates as carbon sources from a Bacillus sp. BCRC 10094. The maximum CGTase activity was about 1400 unit/ml in a shaking culture using 2%(v/w)corn starch, cassava starch, soluble starch (Kanto Chemical Co. Ltd) at 33℃and 160 rpm. The carbon sources using DE10, DE20 soluble starch (Taiwan Fructose Co.,Ltd) and maltose in the medium produced less CGTase activity. The CGTase of this study and commercial CGTase (NOVO) were employed to produce CDs from 15 % (w/v) starch. CDs were produced from corn starch catalyzed by CGTase (NOVO, 250 unit/g-starch) at 75 C for 24 hrs. The yield of CDs from starch was 33 %. However, it was 1000 ~ 2000 unit needed per gram of starch for the CGTase cultured from Bacillus sp. BCRC 10094 in this study. The yield of CDs from starch was 18 ~23 % using our enzyme. The results indicated that CGTase from NOVO had better thermal stability.
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Kow-Jen Duan |
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Kow-Jen Duan Gen-How Chen 陳鈞浩 |
author |
Gen-How Chen 陳鈞浩 |
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Gen-How Chen 陳鈞浩 Production of cyclodextrin using cyclodextrin glycosyltransferase and different starch |
author_sort |
Gen-How Chen |
title |
Production of cyclodextrin using cyclodextrin glycosyltransferase and different starch |
title_short |
Production of cyclodextrin using cyclodextrin glycosyltransferase and different starch |
title_full |
Production of cyclodextrin using cyclodextrin glycosyltransferase and different starch |
title_fullStr |
Production of cyclodextrin using cyclodextrin glycosyltransferase and different starch |
title_full_unstemmed |
Production of cyclodextrin using cyclodextrin glycosyltransferase and different starch |
title_sort |
production of cyclodextrin using cyclodextrin glycosyltransferase and different starch |
publishDate |
2005 |
url |
http://ndltd.ncl.edu.tw/handle/28752290688455045548 |
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