| Summary: | 碩士 === 國立陽明大學 === 生物化學研究所 === 93 === Based on our previous studies, Arabidopsis AtTLPs may play important roles in ABA signaling and ubiquitin-mediated proteolysis (Lai, et al., 2004). However, the functions of Tubby-like proteins in plants have not been fully understood. Rice is one of the most important staple crops in the world. Of the eleven OsTLP (Oryza sativa Tubby-like protein) genes in rice, most have a conserved Tubby domain and F-box domain in their C- and N-termini, respectively. Bioinformatic prediction reveals several possible physiological functions of OsTLPs. Further the prediction by RT-PCR analysis showed that the expressions of OsTLP1, 7, and 11 were upregulated under multiple biotic and abiotic stresses, including abscisic acid (ABA), methyl jasmoic acid (MeJA), salicylic acid (SA), and salt, especially in the roots. During seed development and germination, the expression of OsTLP1, 7, and 11 were upregulated in seed desiccation and downregulated in imbibition, as well as LEA (late embryogenesis abundant proteins). The data all suggests that OsTLPs may play important roles in stress response in rice. In addition, according to the transient assay in rice callus by bombardment, the leader intron of OsTLP7 promoter may function as a transcriptional or translational enhancer to enhance the GUS activity by 8-10 fold. It may be valuable to study whether the intron also enhances other heterogenous promoters to a higher expression level.
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