Studies on Manufacturing Process of Hinoki Oil and its Antibacterial Activity

• Main Authors: Chia-Huei Lee, 李加惠
• Other Authors: Fu-Shung Liu
• Format: Others
• Language: zh-TW
• Published: 2005
• Online Access: http://ndltd.ncl.edu.tw/handle/23407473028543036847

碩士 === 國立雲林科技大學 === 工業化學與災害防治研究所 === 93 === Essential oil from plants usually contains a variety of natural aromas which either attract or repel other hostile organisms. Many papers on study of wood bioactivity were published overseas, much less domestic scientists were involved in the investigation of the antibacterial activity of essential oils of wood such as hinoki. This research utilizes different extraction methods to extract the hinoki oil and to investigate the antibacterial activity of essential oils of hinoki grown in mainland China. The content of effective components of the hinoki oil was analyzed by gas chromatography-mass spectrometry (GC-MS). Regarding colour problem of hinoki oil, two methods namely double extraction and activated carbon methods were utilized. The discussion finally centered on effectiveness of its antibacterial activity and the minimum inhibitory concentrateon（MIC）. Hinoki oil yield decreased according to the extraction method used: soxhlet extraction > solvent distillation extraction > cold solvent extraction > double extraction > Enfleurage. The extraction solvent used affects the yield: Methyl alcohol > Ethanol > Acetone > Hexane > Water. It was found that extraction of hinoki oil mostly can be done in 3 hours. Utilization of GC-MS analysis revealed that it can identify 24 kinds of chemical compounds in hinoki oil, with terpenes as major components. Process varies in its ability in extracting effective composition: soxhlet extraction > solvent distillation extraction > double extraction > cold solvent extraction. Effectiveness of color removal is in the order of: double extraction > activated carbon adsorption. In the antibacterial test, antibacterial activity of hinoki oil towards six bacterial strains is in the order of： Bacillus subtilis > Bacillus cereus> Escherichia coli > Aeromonas hydrophila > Proteus vulgaris > Serratia marcescens. The observation also revealed that hinoki oil can remove Gram-positive bacteria more easily than Gram-negative bacteria. The concentration of hinoki oil in 20 ppm is already enough to show inhibitory effect.