Transport of Stavudine、Delavirdine and Saquinavir across an In Vitro model of the Blood-Brain Barrier by PBCA、MMA-SPM Nanoparticles and SLN

• Main Authors: Fu-long Su, 蘇福隆
• Other Authors: none
• Format: Others
• Language: zh-TW
• Published: 2005
• Online Access: http://ndltd.ncl.edu.tw/handle/47467352543949192451

碩士 === 國立中正大學 === 化學工程所 === 94 === Transport study of stavudine (D4T), delavirdine (DLV) and saquinavir (SQV) by polybutylcyanoacrylate (PBCA) nanoparticles (NPs), methylmethacrylate-sulfopropylmethacrylate (MMA-SPM) nanoparticles and solid lipid nanoparticles (SLN) across the in vitro blood brain barrier (BBB) model system was presented and investigation loading efficiency (LE) of there the durgs onto the three NPs. At the same time for similar human state and accorder with the tissue engineering concept, through human brain microvessel endothelial cells (HBMECs) were cell lines by subculture technique. The drugs LE on PBCA and MMA-SPM series : D4T > DLV > SQV; D4T loaded on NPs series : MMA-SPM > PBCA, DLV and SQV loaded on NPs : PBCA > MMA-SPM; LE decreased following by the diameter of PBCA and MMA-SPM NPs increased. The drugs entrapment efficiency (EE) in SLN series : SQV > DLV > D4T, SLN of diameter enhanced following by the EE. The drugs permeabilities series: D4T > SQV > DLV; the drugs permeabilities enhanced across the BBB by NPs series: PBCA > SLN>MMA-SPM, permeabilities decreased following by the diameter of PBCA and MMA-SPM NPs increased ; SLN is opposite. In addition, PBCA and MMA-SPM under the same diameter LE enhanced following by the carrier amount increase , but does not influence the BBB permeability; Use different Proportion of tripalmitin and cacao butter formate as internal phase synthesized SLN, DLV and SQV EE decreased following by cacao butter amount increased, D4T on the contrary, and store the steady degree of particle after six weeks well under 4 degrees Centigrade of environment.