Effect of Calcium Ion, Potassium Ion and Human Fibronectin on the Cultivation of Bovine Knee Chondrocytes in the PEO-Chitosan Scaffold

• Main Authors: Ya-Ju Hsu, 雀悖
• Other Authors: Yung-Chih Kuo
• Format: Others
• Language: zh-TW
• Online Access: http://ndltd.ncl.edu.tw/handle/36916359356164661421

碩士 === 國立中正大學 === 化學工程所 === 94 === Polyethylene oxide (PEO) and chitosan were hybridized in various weight percentages by genipin crosslinkage under prefreezing temperature of -80℃ to form tissue-engineering scaffolds via lyophilization. And the scaffolds were coated by human fibronectin (HFN) on the surface and added growth factor of TGF-β1 in the Dulbecco’s modified eagle’s medium (DMEM) for the culture of bovine knee chondrocyte (BKC). In addition, the concentrations of calcium ion and potassium ion in the DMEM were changed in various conditions. The experiment results revealed that the pore diameter of PEO-chitosan scaffold were from 200μm to 400μm, a higher weight percentage of PEO in the PEO-chitosan scaffold would yield a greater porosity, a larger moisture, a lower Young’s modulus and compressive modulus, and a higher ductility. The amount of chondrocytes, glycosaminoglycans (GAG) and collagen increased by the listed conditions: (1) An increase in the PEO percentage. (2) An increase in the calcium ion concentration. (3) The PEO-chitosan scaffold coated by HFN. However, an increase in the potassium ion concentration generated negative effects on the cell culture. The results showed that the porous scaffolds could make chondrocytes grow and synthesize extra cellular matrices (ECM) in the experiment conditions.