Fiber-Optic Biosensors Using Light Scattering of Gold Nanoparticles
碩士 === 國立中正大學 === 化學所 === 94 === A novel light-scattering sensing platform was constructed on the basis of modification of the unclad portion of an optical fiber with self-assembled gold nanoparticles. When excited with a Diode Pumped Laser at 532 nm, the light-scattering signal from the optical fib...
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ndltd-TW-094CCU050650422015-10-13T10:45:18Z http://ndltd.ncl.edu.tw/handle/20083728671412157939 Fiber-Optic Biosensors Using Light Scattering of Gold Nanoparticles 無 yawei chu 邱業瑋 碩士 國立中正大學 化學所 94 A novel light-scattering sensing platform was constructed on the basis of modification of the unclad portion of an optical fiber with self-assembled gold nanoparticles. When excited with a Diode Pumped Laser at 532 nm, the light-scattering signal from the optical fiber changes with different refractive index of the environment near the gold nanoparticle surface. With sucrose solutions of increasing refractive index, the light-scattering signal increases linearly. The sensor resolution is 4.5*10-2 RIU. This change in the light-scattering property of gold nanoparticles in solutions of different refractive indexes has been confirmed by simulations. To increase the selectivity of the sensor, we chose three receptor-analyte pairs, biotin/streptavidin, biotin/anti-biotin, and DNP/anti-DNP, to test the sensor. With excitation by a He-Cd laser at 442 nm, results show that the light-scattering signal increases linearly with increasing concentration of each analyte. With biotin modified on the gold nanoparticle surface, the limit of detection (LOD) of the sensor for streptavidin was 8.4*10-9M. To increase sensitivity of the sensor, the sensor length was increased from 1cm to 4cm. As such, the LOD of the sensor for streptavidin lowered to 3.6*10-10M. Using a sensor length of 4cm, the LOD of the sensor for anti-biotin and anti-DNP were 6.3*10-8M and 2.9*10-9M, respectively. The effect of excitation wavelength on the sensing platform was also explored. Using excitation wavelength of 532nm instead of 442nm, the LOD of the biotin-functionalized sensor for streptavidin was 3.4*10-9M Lai-kwan Chau 周禮君 2006 學位論文 ; thesis 68 zh-TW |
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碩士 === 國立中正大學 === 化學所 === 94 === A novel light-scattering sensing platform was constructed on the basis of modification of the unclad portion of an optical fiber with self-assembled gold nanoparticles. When excited with a Diode Pumped Laser at 532 nm, the light-scattering signal from the optical fiber changes with different refractive index of the environment near the gold nanoparticle surface. With sucrose solutions of increasing refractive index, the light-scattering signal increases linearly. The sensor resolution is 4.5*10-2 RIU. This change in the light-scattering property of gold nanoparticles in solutions of different refractive indexes has been confirmed by simulations.
To increase the selectivity of the sensor, we chose three receptor-analyte pairs, biotin/streptavidin, biotin/anti-biotin, and DNP/anti-DNP, to test the sensor. With excitation by a He-Cd laser at 442 nm, results show that the light-scattering signal increases linearly with increasing concentration of each analyte. With biotin modified on the gold nanoparticle surface, the limit of detection (LOD) of the sensor for streptavidin was 8.4*10-9M. To increase sensitivity of the sensor, the sensor length was increased from 1cm to 4cm. As such, the LOD of the sensor for streptavidin lowered to 3.6*10-10M. Using a sensor length of 4cm, the LOD of the sensor for anti-biotin and anti-DNP were 6.3*10-8M and 2.9*10-9M, respectively. The effect of excitation wavelength on the sensing platform was also explored. Using excitation wavelength of 532nm instead of 442nm, the LOD of the biotin-functionalized sensor for streptavidin was 3.4*10-9M
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author2 |
Lai-kwan Chau |
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Lai-kwan Chau yawei chu 邱業瑋 |
author |
yawei chu 邱業瑋 |
spellingShingle |
yawei chu 邱業瑋 Fiber-Optic Biosensors Using Light Scattering of Gold Nanoparticles |
author_sort |
yawei chu |
title |
Fiber-Optic Biosensors Using Light Scattering of Gold Nanoparticles |
title_short |
Fiber-Optic Biosensors Using Light Scattering of Gold Nanoparticles |
title_full |
Fiber-Optic Biosensors Using Light Scattering of Gold Nanoparticles |
title_fullStr |
Fiber-Optic Biosensors Using Light Scattering of Gold Nanoparticles |
title_full_unstemmed |
Fiber-Optic Biosensors Using Light Scattering of Gold Nanoparticles |
title_sort |
fiber-optic biosensors using light scattering of gold nanoparticles |
publishDate |
2006 |
url |
http://ndltd.ncl.edu.tw/handle/20083728671412157939 |
work_keys_str_mv |
AT yaweichu fiberopticbiosensorsusinglightscatteringofgoldnanoparticles AT qiūyèwěi fiberopticbiosensorsusinglightscatteringofgoldnanoparticles AT yaweichu wú AT qiūyèwěi wú |
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