| Summary: | 碩士 === 中國醫藥大學 === 中國藥學研究所碩士班 === 94 === Abstract
In the present study, we investigated the anti-depressant effect of methanol extract of Coptidiis Rhizoma (CR MeOH)and berberine by using forced swimming test (FST) and tail suspension test (TST). We also investigated the mechanism of action of antidepression of berberine by combining with the desipramine (tricyclic antidepressant), maprotiline (selective NA uptake inhibitor), fluoxetine (selectives erotonin reuptake inhibitors) and moclobemide (monoamine oxidase A inhibitors). Then we further measured norepinephrine, serotonin, dopamine and its metabolites levels in mice striatum, hippocampus and cortex to understand the mechanism of the anti-depressive effect.
The results shown that methanol extract of CR MeOH (0.1, 0.5 g/kg) and berberine (0.02 mg/kg ), significantly reduce the immobility time during the forced swimming test .CR MeOH(0.1, 0.5 g/kg) and berberine (0.02 mg/kg) significantly inhibited immobility time during the tail suspension test.
Berberine could significantly decrease the immobility time induced by desipramine, maprotiline and moclobemide, and did not affect the immobility time induced by fluoxetine.
Futhermore, berberine increased norepinephrine and serotonin level in the hippocampus and cortex and dopamine level in the striatum.
Our findings support the view that CR MeOH and berberine exerts anti-depressive effects. The anti-depressive mechanism of berberine may be related to the increase in norepinephrine and serotonin levels in the hippocampus and cortex, dopamine level in the striatum.
|
|---|
