| Summary: | 碩士 === 中國醫藥大學 === 營養學系碩士班 === 94 === Expression of cell adhesion molecules （CAM）by the endothelium and the attachment of monocytes to endothelium may play a major role in the early atherogenic process. Chlorogenic acid（CA）is a phenolic compound present in coffee, apple, pears, berries, almond, artichoke and aubergines. Previous study indicated that CA possess antioxidant activity in vitro. In this study, the effect of CA on the formation of intracellular reaction oxygen species（ROS）and expression of CAM in IL-1β induced human umbilical vein endothelial cells（HUVEC）were investigated, and compared with the control probucol. Our data showed that the inhibition of LDL oxidation（IC50）of CA and probucol were 3.1 ±0.4 μM and 5.5 ±0.7 μM, respectively. The Scavenging ability of DPPH radical（IC50）of CA and probucol were 36.3 ±2.7 μM and 41.8 ± 3.1 μM, respectively. CA dose-dependently suppressed IL-1β-induced mRNA expression on vascular cell adhesion molecule-1（VCAM-1）, intercellular cell adhesion molecule-1（ICAM-1）and endothelial cell selectin（E-selectin）by real-time PCR analysis. CA also suppressed IL-1β-induced the production of ROS. We observed that CA attenuated or blocked nuclear translocation of nuclear factor-κB（NF-κB）P50, P65, P52, Rel-B and C-Rel stimulated by IL-1β, which in turn attenuated CAM expression at the transcription level. Furthermore, CA significantly reduced the adhesion of humans monocyte cell line, U937, to HUVEC treated IL-1β in a dose-response manner. These results demonstrate that CA has inhibitory effect on proanthersclerotic mechanism in vitro.
|
|---|
