| Summary: | 碩士 === 嘉南藥理科技大學 === 生物科技系暨研究所 === 94 === Mammalian spermatogenesis is a highly organized process consists of mitosis, meiosis and post-meiotic regulation, which requires the structural reorganization of chromatin in germ cells. Histones are major components of chromatin, and the testis-specific or testis-enriched histones are believed to be involved in the differentiation of spermatogenesis. Although a number of histones variants have been identified in mammalian testes, their exact functions are still unclear. In this study, we examined the expression profiling of different histone genes both in mouse and human testes. Using the gene-bank search and literature review, the following genes and their human homologous were tested by way of RT-PCR: Mm.193539, Mm.222733, Mm.8048, Mm.24350, Mm.30482, Mm.261670, Mm.261665, Mm.245931, Mm.283802, Mm.117541, Mm.5220, Mm.249751, Mm.261676,Mm.264645, Mm.352429, Mm.15595, Mm.14775 and Mm.144300. Of the histone genes studies, six were expressed in early stages: Mm.193539, Mm.222733, Mm.261665, Mm.249751, Mm.15595 and Mm.14775, six were expressed in all stages of spermatogenesis: Mm.24350, Mm.283802, Mm.117541, Mm.5220, Mm.261676 and Mm.144300, and two were expressed in late stages: Mm.261670 and Mm.264645. Mm.8048, Mm.30482 and Mm.264645 have been shown to be expressed abundantly in mouse testis rather than other organs. Hs.533293, Hs.226117 and Hs.530461 are expressed abundantly in human testis rather than other organs. The absence or decreased expressions of Mm.193539, Mm.222733, Mm.8048, Mm.30482, Mm.264645 and Mm.352429 genes were found in c-kit knockout mice (lack of germ cell). The mRNA transcript levels of Hs.7644、Hs.533293、Hs.25934、Hs.530461、Hs.477879、Hs.137594、Hs.546314、Hs.534369、Hs.484990、Hs.143080、Hs.248172、Hs.136857、Hs.119192、Hs.553494 were significantly decreased in patients without spermatogenesis. Our results suggest that mammalian spermatogenesis appears to be regulated by organized stage- and cell-specific histone expressions both in the germ cells and the somatic cells.
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