| Summary: | 碩士 === 中山醫學大學 === 生化暨生物科技研究所 === 94 === Diosgenin, a steroidal saponin present in fenugreek (Trigonella foenum graecum) and other plants, has been shown to suppress inflammation, inhibit proliferation, and induce apoptosis in a variety of tumor cells. However, understanding of the molecular mechanism of diosgenin effects on human breast cancer cells is not yet fully clarified. Therefore, the aim of the present study was to investigate the anticancer effect of diosgenin on human breast cancer cell lines, MCF-7 and Hs578. We found that diosgenin effectively induced cell cycle arrest at G2/M stage, associating with reduced expression of cyclin B, cyclinA, Cdc25c in a p53-dependent manner. In addition, diosgenin triggers cell apoptosis through a increase in the protein expression levels of cytohrome c, caspase 9 and caspase 3, whereas is inversely related to Bcl-2 protein expression. Moreover, Fas protein expression was significantly increased according to treatment of diosgenin. As a result, evidences of the mitochondrial membrane potential change and DNA fragmentation assay provide that diosgenin induced apoptosis on breast cancer cell lines. Worthnotingly, Chk2 protein was significantly reduced after diosgenin treatment, which effect might correlate with cdc25c phosphorylation. Cell thus arrest at G2/M stage through inactivation of the complex of cdc2-cyclin B due to the phosphorylated cdc25c. In conclusion, our results may shed light on the certainty that diosgenin is available in the induction of apoptosis on breast cancer cells, providing the development of new anticancer strategy for future therapeutic approaches.
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