Study of Hibiscus Sabdariffa Linnaeus polyphenols and anthocyanins extracts on anti-inflammation

• Main Authors: Shu-Hui, 楊淑慧
• Other Authors: 李彗禎
• Format: Others
• Language: zh-TW
• Published: 2006
• Online Access: http://ndltd.ncl.edu.tw/handle/24211729240918537352

碩士 === 中山醫學大學 === 生化暨生物科技研究所 === 94 === In Taiwan, Hibiscus Sabdariffa Linnaeus (HS), a well-known beverage, was demonstrated to represent antioxidant effect in previous studies. To further exploit the extracts, polyphenols and anthrocynins from HS (HPE and HA), we investigated the anti-inflammatory of effect of target sample in RAW264.7 cell on inflammatory mediators secretions (Nitrite and PGE2). Sequentially, animal model study was also preformed to confirm the anti-inflammatory effect of HPE and HA on lipopolysaccharide (LPS)-induced acute hepatic inflammation. The results showed that HPE and HA (0.01-0.5mg/ml) reduced the xanthine oxidase activity to 94.6% and 10.5% in the highest concentration in vitro, and also decreased the nitrite and PGE2 secretion in LPS-activated cells. The in vivo assay showed that pretreatment of HPE (10-40 mg/kg) or HA (20-60 mg/kg) for 5 days significantly decreased the levels of AST and ALT induced by LPS. Therefore also evaluate the antioxidant enzyme or antioxidant molecule, such as catalase and glutathione (GSH) and decrease the level of lipid peroxidation in liver. In histopathological evaluation of rat livers, it revealed that HPE and HA reduced the incidence of liver lesion such as inflammatory cell and infiltration induced by LPS. Furthermore, we also found that pretreated with HPE and HA decreased the hepatic protein expressions of COX-2, p-JNK and p-ERK induced by LPS in rats. These results showed that HPE and HA represented anti-inflammatory potential in vitro and in vivo; and they could be developed to nature, non-toxic anti-inflammatory materials.