| Summary: | 碩士 === 中山醫學大學 === 生化暨生物科技研究所 === 94 === Stress is correlated to the pathophysiology of anxiety, and anxiety is the primary response to stress. The pathophysiology mechanism probably via the glutamatergic N-methyl-d-aspartate (NMDA) receptor is involved in stress responses. It has been demonstrated behavioral effect by long- term exposure to D-cycloserine (DCS). However, still remains unknown the physiological mechanisms of DCS.
The aim of this study, Male Wistar rats, were subjected to the elevated plus-maze (EPM) test divided into low anxiety (LA) and high anxiety(HA) subgroup. Rats received a forced swim test, to observe the cognitive and behavioral effects of DCS depend on the depression level. After excision brain striatum of rats, western blotting assay was used to analysis the striatum proteins, and to study relationship between the biochemistry and behavioral with SPSS.
The present results show that high (HA) anxiety rats following increased by DCS-treated, and up-regulated expression of E- and D1-cyclins, resulting in G1/S stage of cell cycle progression. In addition, showed an increasing trend of Bcl2. Moreover, cytochrome C activity will be forced to enhance after DCS-treated. Interestingly, activating apoptotic signaling pathway by membrane Fas-receptor , and leading to the activation of the downstream caspases, including caspase-3.
In this study, we established an animal''s anxious behavior model with the administration of DCS (5, 10, or 30 mg/kg i.p.) or saline. In conclusion, this were probing into expression of difference to the striatum proteins in brain, but also contributes to an important information to clinical therapy.
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