Effects of NFIL3 on Zebrafish Embryonic Hematopoiesis

• Main Authors: Qi-Zhang, 蘇啟彰
• Other Authors: Zhe-Yu Liu
• Format: Others
• Language: zh-TW
• Published: 2006
• Online Access: http://ndltd.ncl.edu.tw/handle/14544189600779532370

碩士 === 中山醫學大學 === 生化暨生物科技研究所 === 94 === NFIL3（nuclear factor,interleukin 3 regulated）this transcription factor is a member of the bZIP （Basic leucine zipper）family of DNA-binding proteins that display an unusual DNA binding specificity.NFIL3 was found in many various species. It was known that NFIL3 was up-regulated by the IL-3 signaling pathway and plays an important role in the anti-apoptosis response of IL-3. In this study, zebrafish was selected as an animal model to analyze the roles of NFIL3 in zebrafish embryogenesis. The temporal expression of NFIL3 in zebrafish was analyzed by RT-PCR and whole-mount in situ hybridization. The specific siRNA for zebrafish NFIL3 was microinjected into zebrafish one-cell embryos and it was found that the embryonic heart development and heart function was abnormal, the embryogenesis was delayed and the hematopoiesis was decreased when staining by O-dianisidine. In a word that NFIL3 could be related to heart development and hematopoiesis in zebrafish.