| Summary: | 碩士 === 義守大學 === 生物技術與化學工程研究所碩士班 === 94 === The inorganic molecularly imprinted polymer (MIP) was synthesized by the condensation of silicate from tetraethyl orthosilictate (TEOS) under hydrothermal conditions at 60℃. Aluminum chloride was added as a functional monomer to increase the material’s rebinding ability. The selectivity of the MIP receptor prepared with different ratios of template to Si and Al, was examined with seven analytes including: dopamine (DA), epinephrine (EP), norepinephrine (NEP), ascorbic acid (AA), homovanillic acid (HVA), uric acid (UA), and l-tyrosine. The results showed a size selective effect for the receptors with respect to the recognition of the catecholamines. Some factors affecting the recognition ability were investigated including: the solution pH of analytes, surface capping on the MIP, and the imprinting pH of the silica–alumina solution. Also, the catecholamine MIP films on quartz crystal microbalance (QCM) electrodes were fabricated as sensors for in situ monitoring of the analytes in a 2-propanol solution.
The organic MIP film was prepared by using polypyrrole with conductive property. The film response to DA binding with a current change (DA is electro-oxidized into o-quinone) caused by applying positive voltage from one side of the recognition film to the other. The recognition film was synthesized by molecularly imprinting DA on polypyrrole coated on an carbon electrode, this was activated for cation adsorption by treatment with NaOH solution. The polymerization of pyrrole was carried out at 110℃ to evaporate the solvent. Determination of the potential windows of following analytes: DA, EP, NEP, and AA were made using the MIP electrode. The results indicated that the current response of the MIP electrode is more specific for DA than EP, NEP, or AA (at 0.44Vvs. Ag/AgCl). Also, the MIP electrode was shown to a higher sensitivity for DA than the non-MIP electrode.
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