| Summary: | 碩士 === 高雄醫學大學 === 藥學研究所碩士班 === 94 === Amphetamine analogues amphetamine, methamphetamine, 3,4-methylenedioxymethamphetamine (MDMA), 3,4-methylene- dioxyamphetamine (MDA) and methylenedioxyethylamphetamine (MDEA) have become popular drugs of abuse in recent year, because they are powerful stimulants of the central nervous system and serious injure to the human body. A simple, rapid and sensitive derivatization and fluorimetric-liquid chromatographic method is described for the determination of amphetamine analogues in human urine. The method is based on the derivatization of the amphetamine analogues from urine samples with (2-naphthoxy)acetyl chloride (NAC). The resulting naphthoxy derivatives were analyzed by liquid chromatography with fluorimetric detection (excitation wavelength 229 nm, emission wavelength 345 nm). Several parameters affecting the derivatization of amphetamine analogues were discussed including the concentration of derivatization reagent, concentration and pH value of borate buffer, reaction temperature and reaction time. The linear range of the method for the determination of amphetamine analogues derivatives were 20 ~ 800 ng/mL. The detection limit of amphetamine analogues derivatives were reach 4 ng/mL (S/N = 3, injected amount 10 ?尳). Application of the method to the analysis of amphetamine analogues in drug abuser’s urine proved feasible.
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