Studies on the Bioactive Constituents from Sapindus mukorossi and Galla Chinensis

• Main Authors: Hui-Chi Huang, 黃慧琪
• Other Authors: Yang-Chang Wu
• Format: Others
• Language: zh-TW
• Published: 2006
• Online Access: http://ndltd.ncl.edu.tw/handle/73185222566263437159

博士 === 高雄醫學大學 === 藥學研究所博士班 === 94 === As a series of studies on the chemical constituents and biological activities (anti-canacer, anti-molluscoid, and anti-bacteria) of Taiwanese plants, the methanolic extracts of the fruits of Sapindus mukorossi and the EtOH extract of Galla Chinensis (the galls of Rhus chinensis) had antibacterial activity and the EtOH extract of the fruits of S. mukorssii showed the effect of causing death of Pomacea canaliculata (Lamarck), as well as the EtOH extract of the galls of S. mukorossi showed anti-platelet aggregation activity and cytotoxicity against several human tumor cell lines. Investigation of the methanolic extract of fruits of S. mukorossi led to the isolation of six new saponins and thirty-five known compounds. The bioassay for preventive activity test against Pomacea canaliculata revealed that Oleanane-type saponins had 70~90 % inhibition at a concentration of 10 ppm. In addition, the oleanane-type saponins also show significant anti-inflammatory activity. Twenty-two new compounds together with thirty-six known compounds were isolated from the galls of S. mukorossii. Biological evaluation showed that tirucallane-type saponins, had potent anti-platelet aggregation [induced by platelet-activity factor (PAF), arachidonic acid (AA), thrombin (THB) and U46619] and no obvious cytotoxicity (measured by LDH leakage, <14.71 %, conc. 30 ?嵱). Subsequent purification of Galla Chinensis yielded twenty-one known isolates including flavanoids, triterpenes, and tannins. The evaluation in anti-bacteria and anti-inflammatory activity for these isolates is still under progress. The structural elucidation of these twenty-eight new and ninety-two known compounds are based on spectroscopic analyses (mainly 1D and 2D NMR techniques), as well as by chemical evidences.