Studies on micropropagation of Echinops grijsii Hance

• Main Authors: Jing-Yu Li, 李璟妤
• Other Authors: Mau-Shing Yeh
• Format: Others
• Language: zh-TW
• Published: 2006
• Online Access: http://ndltd.ncl.edu.tw/handle/05798512934649232250

碩士 === 國立中興大學 === 農藝學系 === 94 === This study is exploring the regenerating condition of tissue culture using different parts of Echinops grijsii Hance ,and estimating the processing of formation ,differentiation and development of somatic embryo or shoot transferring from callus by paraffin method .We are expecting to establish the best condition for accelerating regeneration rate of E. grijsii Hance by techics of tissue culture . The result can be used as a model for considerable regenerating and a consultation for cropping. The results of this experiment are described below: Germfree explant , originated from seeds germinating in MS medium, ware cultured in MS medium added with 12 kinds of medium composed with BA ( 0, 0.5, 1 and 2 mg/L) and NAA ( 0, 0.5 and 1 mg/L ) combination.The number of induced shoots (4.5) under MS + BA 1 mg/L and hight after 1 month cultivation. The second one (2.7) is under MS + BA 0.5 mg/L. The number of the shoots, induced from stem under MS + BA are regenerated in 125 ml glass bottle using same medium, are 4.6 . Then the induced shoots are divided to 1 shoots regenating twice with same medium in glass bottle No. 5. The number of the newly regenerating shoots are 2.8 after one month cultivation. Then regenerating of the shoot again, the number of shoots are 2.6 one month 1ater.Single shoots culting from regenerating shoot are cultured in MS medium added with NAA 0, 0.5, 1, 2 and 4 mg/L for rooting. The rate of rooting with NAA 0.5 mg/L are best (29.2﹪), the number of forming root are 3.4. We can get healthy E. grijsii Hance plant with hardening after one month. The 75﹪ harden plants grow well and blossom now. Leaf and cotyledon from germfree E. grijsii Hance , and floret talcen from plant grown in greenhouse, and premature cotyledon talcen from plant grown in fided, all of the matcrials are cultivated in MS medium with BA (0, 0.5, 1 mg/L) and NAA (0, 0.1, 0.3 mg/L) combination. The callus formation rate from leaf cultivafed with BA 1 mg/L + NAA 0.3 mg/L are highest, 70﹪, and the formation rate of shoot are 10﹪(1.5 shoots , 0.3 cm length) The callus formation rate from cotyledon cultivated with BA 0.5 mg/L + NAA 0.3 mg/L are highest , 95﹪,and the formation rate of shoots are 70﹪(2.9 shoots, 1.6 cm length).The callus formation rate from flowret cultivated in BA 0.5 mg/L + NAA 0.1 mg/L , BA 1 mg/L +NAA 0.1 mg/L and BA 1 mg/L + NAA 0.3 mg/L are highest, 100﹪,and the formation rate of shoots with BA 0.5 mg/L + NAA 0.1 mg/L and BA 0.5mg/L + NAA 0.3 mg/L are 70﹪(1.57 shoots, 1.86 shoots, separately).The formation rate of shoots from premature flowret with BA 0.5 mg/L + NAA 0.1 mg/L are 35﹪(1.14 shoots, 1.5 cm length), and the formation rate of somatic embryo with BA 0.5 mg/L + NAA 0.1 mg/L are highest 35﹪. The second one which are under BA 1 mg/L + NAA 0.3 mg/L is 15﹪. The arranglment of the cells of explants after 2 months cultivation are observed. The abilith of differentiation of the callus induced frim leaf is not good. The cells of callus, induced from cotyledom, are smaller and forming tight and getting ability to differentiating into shoot primodium for formation of shoots. Explants from flowret areobserved to show a large dividing cell groups. This cell groups have ability of differentiation to produce shoot primodium.The callus from the premature cotyledon have the ability of differentiation into somatic embryo and shoot primodium.