| Summary: | 碩士 === 國立中興大學 === 化學工程學系所 === 94 === Preparations of biosensors based on the MWCNT/Nafion/glucose oxidase (GOD) coated on the thin film glassy carbon electrode (GCE) for detecting the glucose and hydrogen peroxide (H2O2) were investigated. Different methods such as electrochemical reduction, electrochemical deposition, physical adsorption, blending＆ prereduction（BP）were used to immobilize the gold nanoparticles onto the MWCNT/Nafion of GCE biosensor (Au/MWCNTs/Nafion/GCE), which could be applied in detection of hydrogen peroxide and glucose concentrations.
With the operating votage of -0.44V, it showed the highest current of 8.37μA with the accumulation of 1.07mM H2O2 was observed when using BP approach. In this study, BP approach could give the highest current, therefore it could be further applied to the detection of glucose concentrations. Under the optimal conditions of 1mg/ml GOD, 20μl gold solution, pH7 and agitation speed of 300rpm, the sensitivity and response time of Au/GOD/MWCNT/Nafion/GCE biosensor were 0.0043 mM and 8 seconds respectively in the detecting range of 0.0043 ~ 10.07mM glucose.
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