Using cDNA-AFLP to Understand the Relationship among External, Physiological, and Gene Expressions of Camphor Tree Seedlings under Different Light Intensities

• Main Authors: Ying-Ping Huang, 黃盈屏
• Other Authors: 許博行
• Format: Others
• Language: zh-TW
• Published: 2006
• Online Access: http://ndltd.ncl.edu.tw/handle/63399540522534240137

碩士 === 中興大學 === 森林學系所 === 94 === We perform the experiment with camphor tree (Cinnamomon camphora) to understand the overall expressions of the seedlings treated with different light intensities (2%, 25%, and 100% of full light). The results including the external and physiological expressions show that the growth and photosynthesis of the seedlings under the condition of 25% full light are the best. And we use the cDNA-AFLP technology to compare the variation of gene expression between treatments. About 25 obvious fragments of differential expressed gene using 5 primer pairs are eluted from gel, cloned, and sequenced. These sequences are compared and aligned with those sequences deposited in the database of Genebank and could matched to 9 interesting functions. These functions can also be grouped into 4 groups: (I) structure of light harvesting complex related: PSII reaction center protein6.1KDa, PS II reaction center protein 10 kDa (PsbR), transfer RNA-Asp, transfer RNA-Tyr, transfer RNA-Glu, transfer RNA-Ser, and PSII protein (psbI) genes; (II) carbxylation related: transketolase, galactinol synthase, rubisco, and polysaccharide biosynthesis precursor; (III) proteolysis related: ubiquitin-conjugating enzyme; and (IV) regulators: cytochrome P450 like-TBP. However, how do these differential expressed genes involve in the effects on the growth and development of plants is still needed to be further studied.