| Summary: | 碩士 === 國立中興大學 === 生物產業機電工程學系所 === 94 === In this research，the Near Infrared Spectroscopy (NIR ) was used to scan the spectrum of Sulfamethazine (SMT) in duck''s liver powder. Measuring the reflection spectrum of SMT in duck''s would help to evaluate the limit of measuring the low-concentration for self-developed NIR system. Multiple linear regression (MLR),modified partial least squares regression (MPLSR), partial least squares regression (PLSR), principal component regression (PCR) were applied to proceed regression analysis of the spectrum, prediction versus real concentration values. There were 70 types of concentration of sample varied from 0.001 ppm to 10 ppm in this research. Four key wavelengths had chosen from the results to develop the calibration equation. Furthermore, the MPLSR analysis method produced the best result and shown as followings.
The calibration coefficient of determination (Rc2) = 0.864, the standard error of calibration (SEC) = 1.206,and the relative standard error of calibration (RSEC) = 1.978% for the calibration sets. The coefficient of determination of predition (Rp2) = 0.868, standard deviation=3.125, the standard error of predition (SEP) = 1.201,the relative standard error of predition (RSEP) = 2.12%, and ratio of standard deviation to SEP (PRD) = 2.811 for the predition sets. This research verified the evaluating limit of concentration for the self-developed NIR system was 1ppm.
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