| Summary: | 碩士 === 國立中興大學 === 農藝學系所 === 94 === The objectives of this study were to establish a generation system for mass propagation of Cirsium japonicum DC. and C. albescens Kitamura.
Chromosome numbers of in vitro regenerated were also inspected. Three types of explants were used for regeneration and the highest shoot number was obtained in MS basal medium containing with 0.2 mg/L BA and 0.1 mg/L for stem-node and leaf explants of C. japonicum DC. and C. albescens Kitamura under the light. Explants of tubular flowers of C. japonicum DC. cultured in the same basal medium containing with 1.0 mg/L in combination with 0.1 mg/L NAA; or with 4.0 mg/L BA in combination with 0.5 mg/L BA, for had only few shoots (1.1-1.8) regenerated, however C. albescens Kitamura regeneration shoots were found in a medium of 0.2 mg/L BA in combination with 0.5 mg/L NAA and a medium of 0.5mg/L BA in combination with 0.5 mg/L BA. Although a much less or no generated shoots was found in the lightness for all types of explants for both C. japonicum DC. and C. albescens Kitamura, it was found that in the formation of callus was enhanced.
The in vitro regenerated shoots were tested for root formation; highest root formation rates were obtained in a half or full strength of MS medium containing with 0.1 mg/L NAA. The rooted shoots were transplanted directly to the greenhouse.
Chromosome number from root tip cell of in vitro generated plants of C. japonicum DC. and C. albescens Kitamura were inspected and the identical chromosome numbers were found for in vitro and wild plants with the number of 2n=34. The length of chromosomes of C. japonicum DC. was ranged from 8.17-4.29 μm and 9.20-4.81 μm for C. albescens Kitamura. Comparison of the karyotypes form these two species, it is considered that C. japonicum DC. and C. albescens Kitamura are two independent species.
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