| Summary: | 碩士 === 國立成功大學 === 生物科技研究所碩博士班 === 94 === Multipotent stem cells can be applied in clinical medicine for gene therapy and cell therapy. Stem cells have great promise as tools for regenerative medicine, tissue engineering and pharmaceutical research. Human mesenchymal stem cells (MSCs) have been isolated and identified from various adult and fetal tissues. In this investigation, we isolated mesenchymal stem cells from termed placenta. The placenta-derived mesenchymal stem cells exhibit fibroblast-like morphology and express ABCG2, CD29, CD44, CD73, CD90 and CD105 but not SSEA-4, HLA-DR, CD18, CD31, CD45, CD117, CD133. These cells have multilineage potential because they were able to differentiate into cells of adipogenic and osteogenic lineages. The placenta-derived MSCs were also demonstrated to express the mRNA of HOX-b4, Bmi-1, Oct-4, and Rex-1 by reverse transcription PCR.
The population of cells flushed from placenta was further investigated by flow cytometry and only about 1% population was found to be positive for CD90. Through double immunofluorescent staining and confirmation by fluorescent activated cell sorting of the CD90+ cells, they were found to co-express with ABCG2, CD9, CD29, CD73, and CD105, but only part with CD44. It is possible that the subpopulation of CD90+/CD44+ cells could be regarded as one primitive population of MSCs from placenta.
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