| Summary: | 碩士 === 國立成功大學 === 環境工程學系碩博士班 === 94 === This study evaluate microbial ecology and performance of nitrification of active sludge in different municipal and swine waste water treatment plant. Besides of traditional method to investigate characteristic of waste water, performance of nitrification and dynamic nitrification experiment of active sludge, this study used molecular biology techniques - terminal restriction fragment length polymorphism(T-RFLP) to investigate population transfer of ammonia oxydizing bacteria(AOB) and nitrite oxydizing bacteria (NOB).
The subjec this syudy investigate contain a municipal waste water treatment plant and a swine waste water treatment plant. Ammonium in swine waste water inflow has reached to 215~245 mg N/L, and nitrate in outflow has reached to 180~210 mg N/L. Nitrite accumulation in outflow has reached to 5~15 mg N/L with totally nitrification effency more than 70%. TKN in municipal waste water inflow is only one of ten in swine waste water inflow. Most of TKN is ammonium with 60~80% nitrification transform efficiency. No obvious nitrite accumulation in municipal waste water outflow.
T-RFLP in this study compose of 16S rDNA and fuctional gene amoA (Park et al., 2002). In 16S rDNA based T-RFLP result, Nitrosomonas is dominant AOB in municipal and swine waste water treatment without significant Nitrosospira signal. Long term dominant NOB in this two waste water treatment are Nitrobacter and Nitrospira.
In amoA based T-RFLP result, Nitrosomonas europaea is long term dominant in swine waste water treatment. Besides of Nitrosomonas europaea, amoA based T-RFLP detected signal of Nitrosospira, N. communis and N. cryotolerans which exist in high nitrogen condition in swine waste water treatment. Nitrosomonas oligotropha is long term dominant AOB in municipal waste water treatment with low nitrogen condition comparing with swine waste water treatment.
Moreover, this study used cloning-sequencing experiment to investigate AOB in municipal and swine waste water treatment. In cloning-sequencing results, Nitrosomonas europaea is dominant AOB in swine waste water treatment, and Nitrosomonas oligotropha and Nitrosomonas sp. Nm143 lineage is dominant AOB in municipal waste water treatment.
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