development and application of new cationic liposome in vitro/in vivo gene delivery

• Main Authors: Yu-chi Li, 李毓琪
• Other Authors: Ja-an Annie HO
• Format: Others
• Language: zh-TW
• Published: 2006
• Online Access: http://ndltd.ncl.edu.tw/handle/32111509197551228555

碩士 === 國立暨南國際大學 === 生物醫學科技研究所 === 94 === Abstract The development of Drug Delivery System (DDS) is to pursue a potent, efficient and safer condition in drug (or gene) delivery. In DDS, non-viral vector “liposome” is widely studied, not only due to its lipid bilayer structure which is similar to cell membrane, but also its characteristics with biocompatibility and biodegradability. Cationic liposome (with positive charge on the surface) enables to carry negatively charged materials such as nucleic acid, and it is able to interact with negative charged plasma membrane. 　　The goal of this study is to develop a new formula of cationic liposome as gene carrier, which consists of DPPC：DPPE：Cholesterol with molar ratio of 10： 0.075：10. This newly formulated liposome was used to transfect EGFP plasmid DNA into three different cell lines (NIH-3T3, HeLa, and CHO). In addition, the transfection efficiency of DPPE-rich liposome in vivo with reporter gene of pRL-CMV plasmid DNA was also discussed in this study.