Fabrication of Microfluidic Channel for 3T3-L1 Adipocyte cells detection

• Main Authors: Cheng kung yo, 鄭光佑
• Other Authors: Wu yo lin
• Format: Others
• Language: zh-TW
• Published: 2006
• Online Access: http://ndltd.ncl.edu.tw/handle/30176725665123821220

碩士 === 國立暨南國際大學 === 生物醫學科技研究所 === 94 === Microfluidic chips developed for biochemistry applications have been progressed and advanced continuously. With the technological advances and improvement on chip fabrication, researchers now can do the bio-analysis of all the mix, separation and detection procedures in one miniaturized chip. The operation not only decreases the possible external contamination and amount of agents for testing, but also increases the detection efficiency and sensitivity. The main purpose of this thesis is to fabricate a microfluidic chip for triacylglycerol detection by taking the advantage of the photolithography process of semiconductor fabrication technology. The enzymatic transesterification of adipocyte yields triacylglycerol. Lipase can further react with triacylglycerol to produce hydrogen peroxide through hydrolysis. Blue luminescence will be emitted when the agent of chemiluminescence, Luminol, reacts with this hydrogen peroxide generated. In this thesis, we simulated this chemiluminescence process by using the microfluidic chip fabricated. The master for the microfluidic chip was first fabricated by using the thick photoresist, SU-8, followed by molding the microfluidic channel by using macromolecular polymer, PDMS. Then, adipocyte cells were cultivated in the microfluidic chip. We found that the adhesion of adipocyte cell to the fabricated chip was very good during cell cultivation. Therefore, the fabricated chip was suitable for cell growing. Two different colored inks were injected into the microfluidic channel to simulate the function of the microfluidic chip. The chemiluminescence of the chip was also verified in this work by recording the luminescence emitted with a digital camera.