| Summary: | 碩士 === 國立交通大學 === 生物科技系所 === 94 === Recently, there is increasing interest in finding natural antioxidants from plants, which protect human body against free radicals insult and ameliorate the progress of many chronic disease. Polysaccharide, in particular , is supposed to be responsible for some healthy properties of mushrooms. In this study, the ultimate goal was to establish a method for analyzing antioxidant activities on chemical and biological system of polysaccharide from Lentinula edodes culture broth filtrate. Ten isolates of Lentinula edodes , including L1 , L4 , L6 , L10 , L11 , L15 , L21 , L23 , L24 and L25 , were investigated .The antioxidant assays include: reducing power, α, α-diphenyl-β-picrylhydrazyl (DPPH) radical scavenging effect, Fe2+ chelating power and the antioxidant activity by the conjugated diene method. The Lentinula edodes culture broth filtrate result showed that L21 had the best antioxidant active (81.66%) and chelating effect on ferrous ions (82.25%).The reducing powers were good at L15 (0.88). The DPPH scavenging effect was good at L6 (75.75%). The breakthough of this study was to build up an intracellular biological antioxidant model.This study tryied to use hydroxyl peroxide to animate biological oxidative system and discuss the repair power and the protection power. The Clone-9 CRL-1439 were as used model to understand the complicated expression of the intracellular antioxidant enzyme.The result should that the protection power was more significant the repair power. The study of repair Power Indicates L15 and L25 having better performance in high concentration, about the same with VIt C and Vit E. The study of protection Power showed the expression of L25 was better than L15, In high polysaccharide concentration the L25 was remarkable to have better protection ability , even better than Vit C and VIt E.Culture broth filtrate also has good protect performance. To summarize, this syudy truly built up an intracellular biological antioxidant model and showed that both culture broth filtrate and hot-water extract of polysaccharide were antioxidantive in chemical system and biological system.
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