Octylphenol regulation of resistin in 3T3-L1 adipocyte

• Main Authors: Min-Hua Wu, 吳敏華
• Other Authors: Yung-Hsi Kao
• Format: Others
• Language: zh-TW
• Published: 2006
• Online Access: http://ndltd.ncl.edu.tw/handle/45045468320513916131

碩士 === 國立中央大學 === 生命科學研究所 === 94 === Resistin (Rstn) is an adipocyte-specific secretory hormone that can reduce the insulin-stimulated glucose uptake by adipocytes, resulting in insulin resistance, and link the obesity and type II diabetes. It can be regulated by many hormones, but the exact mechanisms of their action is still not clear. Environment hormones are derived from the metabolites of many environmental chemical compounds and have the biological activity in organisms. Octylphenol (OP) is one of environmental estrogens that has the structural similarity to estrogen and can mimic the action of estrogens. In this study using 3T3-L1 adipocytes as the research cell line, OP induced increases in Rstn mRNA and protein expression with significantly dose- and time-dependent differences was discovered. Also, pretreatment with either transcriptional inhibitor actinomycin D or translational inhibitor cycloheximide did not prevent the OP-stimulated Rstn mRNA and protein expression. This suggests that OP increases the stabilities of Rstn mRNA and protein. In addition, pretreatment with either estrogen receptor alpha (ER��) inhibitor ICI182780 or MEK1 inhibitor U0126 prevented the OP-induced increases in Rstn mRNA and protein expression, suggesting that the effect of OP requires the functional ER�� and MEK1. Moreover, OP increased PPAR�� (a negative regulator of Rstn) protein expression and the association of ER�� with PPAR��. Further studies to demonstrating whether the effect of OP on Rstn gene expression is mediated directly via modulation of PPAR�� is needed.