| Summary: | 碩士 === 國立嘉義大學 === 獸醫學系研究所 === 94 === The purpose of this study was to determine the Ornithobacterium rhinotracheale infection and the data of epidemiology in turkeys in Taiwan. We collected laryngeal swabs from commercial turkey farms in different areas and trachea and lung samples from turkey slaughterhouses in Taiwan since Aug 2004 to March 2006 for bacteriological culture and identification. The antimicrobial susceptibility test and 16S rRNA sequence analysis of O. rhinotracheale isolated from turkeys were performed. For investigation of the seroprevalance, blood samples were collected from different commercial reared farms and slaughterhouses for examination antibodies by ELISA. O. rhinotracheale were isolated and identified from turkey laryngeal swabs and trachea and lung samples. The percentage of O. rhinotracheale isolation from laryngeal swabs, trachea and lung samples among Aug 2004 to March 2006 were 6.62 % (62/936), 2.83 % (35/1238) and 1.14 % (14/1224), respectively. The rate of isolation from 4 months old turkeys were higher than others. The percentage of isolation from turkey slaughterhouses was highest in May 2005. Most strains were sensitive to bacitracin (94.68 %), amoxicillin(89.23 %), ampicillin (78.72 %), penicillin G (61.7 %) and ofloxacin (58.51 %). A majority of isolates were resistant to colistin (97.87 %), lincomycin (96.81 %), amikacin (94.49 %), tylosin (91.49 %), erythromycin (89.36 %), gentamicin (89.36 %), streptomycin (87.23 %) and sulphamethoxazole/trimethoprim (SXT; 84.04 % ). The seroprevalance of commercial reared turkeys and flocks were 43.29 % (1693/ 3910) and 76.71 % (168/219), respectively. This is the first report about isolation of O. rhinotracheale from turkeys in Taiwan. These results show that O. rhinotracheale infection is widespread in turkeys in Taiwan, and 16S rRNA sequence analysis show a high similarity among most of O. rhinotracheale isolates might be the same cluster.
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