Expression and Purification of Glutamate mutase
碩士 === 國立東華大學 === 應用物理研究所 === 94 === Coenzyme B12 dependent Glutamatemutase catalyzes the reversible interconversion of L-glutamate to 3-methylaspartate. We prepared and purified the E and S components of glutamatemutase from E-coli. By using EPR technique, we did check the activity of glutamatemuta...
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ndltd-TW-094NDHU55040212015-12-16T04:39:02Z http://ndltd.ncl.edu.tw/handle/10719536472987243087 Expression and Purification of Glutamate mutase 麩胺酸轉換脢的表現與純化 Chi-Ju Wu 吳奇儒 碩士 國立東華大學 應用物理研究所 94 Coenzyme B12 dependent Glutamatemutase catalyzes the reversible interconversion of L-glutamate to 3-methylaspartate. We prepared and purified the E and S components of glutamatemutase from E-coli. By using EPR technique, we did check the activity of glutamatemutase and the radicals formed during the reaction of Glutamatemusate with B12. We wish to do the ENDOR to understand the reaction mechanism, the Co-C bond cleavage and the readicals formed during the reaction. Shyue Chu Ke 柯學初 2006 學位論文 ; thesis 66 zh-TW |
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碩士 === 國立東華大學 === 應用物理研究所 === 94 === Coenzyme B12 dependent Glutamatemutase catalyzes the reversible
interconversion of L-glutamate to 3-methylaspartate. We prepared and
purified the E and S components of glutamatemutase from E-coli. By using
EPR technique, we did check the activity of glutamatemutase and the
radicals formed during the reaction of Glutamatemusate with B12. We wish
to do the ENDOR to understand the reaction mechanism, the Co-C bond
cleavage and the readicals formed during the reaction.
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author2 |
Shyue Chu Ke |
author_facet |
Shyue Chu Ke Chi-Ju Wu 吳奇儒 |
author |
Chi-Ju Wu 吳奇儒 |
spellingShingle |
Chi-Ju Wu 吳奇儒 Expression and Purification of Glutamate mutase |
author_sort |
Chi-Ju Wu |
title |
Expression and Purification of Glutamate mutase |
title_short |
Expression and Purification of Glutamate mutase |
title_full |
Expression and Purification of Glutamate mutase |
title_fullStr |
Expression and Purification of Glutamate mutase |
title_full_unstemmed |
Expression and Purification of Glutamate mutase |
title_sort |
expression and purification of glutamate mutase |
publishDate |
2006 |
url |
http://ndltd.ncl.edu.tw/handle/10719536472987243087 |
work_keys_str_mv |
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