Expression and Purification of Glutamate mutase

Expression and Purification of Glutamate mutase

碩士 === 國立東華大學 === 應用物理研究所 === 94 === Coenzyme B12 dependent Glutamatemutase catalyzes the reversible interconversion of L-glutamate to 3-methylaspartate. We prepared and purified the E and S components of glutamatemutase from E-coli. By using EPR technique, we did check the activity of glutamatemuta...

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Main Authors: Chi-Ju Wu, 吳奇儒
Other Authors: Shyue Chu Ke
Format: Others
Language:zh-TW
Published: 2006
Online Access:http://ndltd.ncl.edu.tw/handle/10719536472987243087
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spelling ndltd-TW-094NDHU55040212015-12-16T04:39:02Z http://ndltd.ncl.edu.tw/handle/10719536472987243087 Expression and Purification of Glutamate mutase 麩胺酸轉換脢的表現與純化 Chi-Ju Wu 吳奇儒 碩士 國立東華大學 應用物理研究所 94 Coenzyme B12 dependent Glutamatemutase catalyzes the reversible interconversion of L-glutamate to 3-methylaspartate. We prepared and purified the E and S components of glutamatemutase from E-coli. By using EPR technique, we did check the activity of glutamatemutase and the radicals formed during the reaction of Glutamatemusate with B12. We wish to do the ENDOR to understand the reaction mechanism, the Co-C bond cleavage and the readicals formed during the reaction. Shyue Chu Ke 柯學初 2006 學位論文 ; thesis 66 zh-TW
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description 碩士 === 國立東華大學 === 應用物理研究所 === 94 === Coenzyme B12 dependent Glutamatemutase catalyzes the reversible interconversion of L-glutamate to 3-methylaspartate. We prepared and purified the E and S components of glutamatemutase from E-coli. By using EPR technique, we did check the activity of glutamatemutase and the radicals formed during the reaction of Glutamatemusate with B12. We wish to do the ENDOR to understand the reaction mechanism, the Co-C bond cleavage and the readicals formed during the reaction.
author2 Shyue Chu Ke
author_facet Shyue Chu Ke
Chi-Ju Wu
吳奇儒
author Chi-Ju Wu
吳奇儒
spellingShingle Chi-Ju Wu
吳奇儒
Expression and Purification of Glutamate mutase
author_sort Chi-Ju Wu
title Expression and Purification of Glutamate mutase
title_short Expression and Purification of Glutamate mutase
title_full Expression and Purification of Glutamate mutase
title_fullStr Expression and Purification of Glutamate mutase
title_full_unstemmed Expression and Purification of Glutamate mutase
title_sort expression and purification of glutamate mutase
publishDate 2006
url http://ndltd.ncl.edu.tw/handle/10719536472987243087
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