Fabrication and Analysis of High Resolution Injection System for Microfluidic Chip

• Main Authors: Yu-Min Zhong, 鍾育民
• Other Authors: Lung-Ming Fu
• Format: Others
• Language: zh-TW
• Published: 2006
• Online Access: http://ndltd.ncl.edu.tw/handle/14713068570045713620

碩士 === 國立屏東科技大學 === 材料工程研究所 === 94 === This study develops a novel capillary electrophoresis (CE) microfluidic device featuring a conventional cross-form injection system and an expansion chamber located at the inlet of the separation channel. The combined injection system / expansion chamber arrangement is designed to deliver a high-quality sample band into the separation channel such that the detection performance of the device is enhanced. Numerical simulations and experimental investigations are performed to investigate the electrokinetic transport processes in the microfluidic device and to establish the optimal configuration of the expansion chamber. The results indicate that an expansion chamber with an expansion ratio of 2.5 and an expansion length of 500 m delivers a sample plug with the correct shape and orientation. With this particular configuration, the peak intensities of the sample are sharp and clearly distinguishable in the detection region of the separation channel. Therefore, this configuration is well suited for capillary electrophoresis applications which require a highly sensitive resolution of the sample plug. This study also designed and evaluated a novel capillary electrophoresis microchip which combines the double-T form injection system with an expansion chamber at the inlet of the separation channel. The novel capillary electrophoresis microfluidic device developed in this study has an exciting potential for use in high-performance, high-throughput chemical analysis applications and in many other applications throughout the field of micro-total-analysis-systems.