| Summary: | 碩士 === 國立屏東科技大學 === 食品科學系 === 94 === The guandu bean or pigeon pea (Cajanus cajan (L.) Millsp.) is a kind of leguminous plant of the Fabbaceae family and is grown in Asia, Africa and South America. With the improvement of the life quality, disease prevention and health care, Cajanus cajan (L.) Millsp. has been paid more attentions for its high content of antioxidant compounds. Per 100-gram seeds include 19.4 g of crude protein, 2.8 mg of zinc, 2.8 mg of iron, vitamin E 7.4 mg, vitamin B1 0.76 mg, and vitamin B2 0.2 mg. Because of its high content of antioxidant compounds, it is one of sources of antioxidant compounds. The objective of this study was to use supercritical fluid carbon dioxide to extract and separate antioxidant compounds from Cajanus cajan (L.) Millsp. Through the orthogonal array experiment (L9) with three factors and levels, the experiment was run under extraction temperature (40, 50, 60°C), extraction pressure (2000, 3000, 4000psi), and extraction time (30, 60, 90min). Results showed that the optimum condition during supercritical carbon dioxide extraction that operated at a pressure of 4000 psi, temperature 60°C, and extraction time 90 min. The evaluation of the antioxidant activities of the extract by supercritical CO2 extraction of optimum condition is also discussed. The antioxidant activities assays, include the α,α-diphenyl-β-picryhydrazyl (DPPH) free radical scavenging effect, effect of chelating ferrous ion, and reducing power, will increase with increasing amount of extract. Extracts were analyzed by using Gas Chromatography equipped with Mass detector. Several compounds were identified, such as terpenes (Squalene), sterols (coprostane, stigmasterol, fucosterol, Lupeol, Tridecanol) and paraffins (Heptacosane, Heneicosane), etc.
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