| Summary: | 碩士 === 國立清華大學 === 分子醫學研究所 === 94 === Planar cell polarity (PCP) is a common feature of many multicellular epithelia and is perpendicular to their apical/basal axis. PCP is manifest in several different body regions and cell types of vertebrates and Drosophila. The conserved PCP proteins called core PCP include frizzled (fz), disheveled (dsh), flamingo (Fmi)/ starry night (stan), prickle (pk), strabismus (stbm)/ Van Gogb(Vang), and diego (dgo). The asymmetric distribution of core PCP provides the correct cues for PCP. In Drsophila eye, PCP is evident in the coordinated orientation of ommatidia that rotate 90° in opposite directions in the dorsal and ventral halves of the eye during eye development. The key point for ommatidia to rotate correctly is the decision of R3/R4 fate that is controlled by PCP.
Echinoid (Ed) is an immunoglobulin (Ig) domain-containing CAMs. Ed negatively regulates EGF receptor signaling pathway during eye development and cooperates with Notch pathway during sensory bristle development. Moreover, we identified that Ed is a component of Adherens Junctions (AJs) that cooperates with DE-cadherin to mediate cell adhesion.
In this report, we show that when ed is mutated, the expression level of Fmi in interommatidia cells is increased and similar to that in ommatidia cells. Furthermore, adult ed mutation eye shows rough phenotype and misrotated ommatidia. Misexpressing Fmi in eye discs also causes Fmi to be increased mainly in interommatidia cells and leads to misrotated ommatidia, similar to the phenotype in ed mutant eye. These results suggest that the regulation of Fmi in interommatidia cells is as important as that in ommatidia cells, and ed may play such a regulation role in this process.
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