| Summary: | 碩士 === 國立臺灣大學 === 化學工程學研究所 === 94 === The goal of this study was to evaluate the effect of dynamic mechanical compression on articular cartilage tissue engineering. We designed a compressive bioreactor and fabricated elastic polyurethane (Pellethane) scaffolds with porosity of 82% and pore size ranged from 200 to 350 µm. Chondrocytes mixed with/without type I collagen hydrogels were seeded into scaffolds. After cultured for 5 days, chondrocyte-scaffold complex was exposed to the uniaxial dynamic compression of 0.1 Hz frequency. The compression of 20% and 30% strain was applied for 0, 4, 8, 12, 24 hours. The other protocol was to culture chondrocyte-scaffold complex for periods of 0, 2. 6, 12, 24 hours respectively after exposed to a 30% strain compression for 12 hours. The cellular response was assessed using RT-PCR assay to quantify expression of type I collagen, type II collagen and aggrecan. We found that the relative intensity of aggrecan was increased with loading duration and maintained for a while after compression, while there was no significant effect on type I and type II collagen gene expression. This system of dynamic compression and elastic polyurethane scaffold may be contributed to the application of engineered cartilage.
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