Design and Synthesis of an Activity Probe Library for Glycosidases

• Main Authors: Sin-Yi Lai, 賴欣宜
• Other Authors: Lee-Chiang Lo
• Format: Others
• Language: zh-TW
• Published: 2006
• Online Access: http://ndltd.ncl.edu.tw/handle/50197113417825870624

碩士 === 國立臺灣大學 === 化學研究所 === 94 === Glycosidases catalyze the hydrolysis of glycosidic linkages in aryl and alkyl glycosides, and occur widely in animals, fungi, plants, and bacteria. These enzymes played crucial roles in numerous biological process as such as cell to cell recognition and interaction, immune response, viral and bacterial infections, and so forth. Dysfunction of their activities often leads to disease conditions. Activity probes can be used to label related enzymes. And the results could potentially be applied to drug development. The concept of activity probes is based on suicide inhibitors of enzymes. A complete activity probe contains four structural units: (1) recognition head; (2) latent trapping device; (3) linker; (4) reporter group. In this thesis, we synthesized a series of activity probes for α-/β-glucosidase, α-/β-galactosidase, α-fucosidase, α-mannosidase and α-xylosidase.