| Summary: | 碩士 === 國立臺灣大學 === 生物產業機電工程學研究所 === 94 === Lignocellulosic biomass has been utilized to produce ethanol in the last two decades. The main process involved in this conversion is hydrolysis of cellulose in the lignocellulosic materials to produce reducing sugars. Due to the recalcitrant nature, the biomass can not be easily converted to monomeric sugars. Therefore, the low yield and high cost of the hydrolysis process are the major challenges in biomass energy development. In our research, marine seaweeds Gracilaria is used as the biomass. Owing to their hydration ability, an “Ice-crystal pretreatment” is developed to enhance the hydrolysis of cellulosic biomass.
The enzymatic hydrolysate of biomass was measured by three methods. Reducing sugars is determined by the dinitrosalicylic method (DNS). Total soluble sugars is determined by the phenol-sulphuric acid method. Glucose is determined by the glucose biosensor. In our result, the validity of “Ice-crystal pretreatment” were confirmed. The effect of “Ice-crystal pretreatment” was also compared with acid pretreatment. In conclusion, marine seaweeds Gracilaria with “Ice-crystal pretreatment” may be a new approach to biomass energy development in the future.
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