| Summary: | 碩士 === 國立臺灣大學 === 醫學工程學研究所 === 94 === Biomaterials are usually applied for growing of cells in tissue engineering. It is very important to understand the interactions of cells with biomaterials. Upon this, hydrophilicity of biomaterials is one of the most important factors in the effects of cell-surface interactions. The purpose of this reserch was to investigate the behaviors of parotid acinar cells on polyvinyl alcohol which is with high hydrophilicity.
Acinar cells were cultured on PVA, and tissue culture plates (TCPS) were used as control group. Functional expression of α-amylase in acinar cells was identified with immuno-fluorescent staining technique. Cell morphology on PVA and TCPS were investigated with light microscopy and scanning electron microscopy (SEM).
Our results demonstrated that in contrast to of attachment of cells on TCPS, cells were suspended and aggregated as spheres on PVA. In the initial stage of cell sphere formation, single cell interface could be identified with SEM. However, after cultured on PVA for a long period, the cell-cell interface was covered with extracellular matrix and cells were hard to identify single cell interface. Immno-fluorescent staining of α-amylase revealed the functional activities of aggregated acinar cells on PVA. Additionally, when receiving the acetylcholine stimulation, these cell aggregates were with higher functional activities compared to those on TCPS.
When aggregated cells on PVA were transferred to TCPS, migration from the aggregates and reattachment on TCPS were evident and cells were also with α-amylase expression.
Our results demonstrated that acinar cells on high hydrophilic biomaterial PVA could be self-assembled as three-dimensional spheres which are more resemble the in vivo state and with better function. We proposed that PVA might be applied for preserving the acinar cells in three-dimensional cell sphere which are more similar to the biological condition in vivo with better function for salivary gland regeneration.
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