Preparation and characterization of silvernanoparticle-doped poly(γ-glutamic acid)/poly(vinyl alcohol)composite hydrogel membrane

• Main Authors: Li-mei Chang, 張麗玫
• Other Authors: Ming-Chien Yang
• Format: Others
• Language: zh-TW
• Online Access: http://ndltd.ncl.edu.tw/handle/5jb6fp

碩士 === 國立臺灣科技大學 === 高分子系 === 94 === An in-situ reduction method was employed to prepare hemocompatible and surface-enhanced Raman scattering (SERS)-active substrates. In this nanocomposite hydrogel membrane, silver nitrate was stabilized with poly(γ-glutamic acid) (γ-PGA).and reduced by polyvinyl alcohol (PVA), thereby resulting silver nanoparticles were doped in PGA/PVA composite hydrogels. Based on the results from Field emulsion scanning electron microscopy (FE-SEM) measurement, the surfaces of nanocomposite hydrogel membrane had well-dispersed spherical silver nanoparticles with an average diameter of 90 nm. It was found that the reduced protein adsorption and platelet adhesion and prolonged blood coagulation time would occur in the presence of PGA. The SERS enhancement of this film had an enhanced factor about 106 for benzoic acid. The cytocompatibility was proven safe by the in-vitro cell cultivation of L929 fibroblasts. Overall results demonstrated that the biomedical silver nanoparticle-doped PGA/PVA composite hydrogel membrane possessed excellent hydrophilicity, antibacterial activity, hemocompatibility and cytocompatibility. Besides, it would be potential as SERS-active substrate for biosensor application.