Identification of Differentially Expressed Messenger RNAs and Proteins in Rat Brain Induced by Repeated Electroconvulsive Shock

• Main Authors: Chih-Ting Huang, 黃芝婷
• Other Authors: Chia-Hsiang Chen
• Format: Others
• Language: en_US
• Published: 2006
• Online Access: http://ndltd.ncl.edu.tw/handle/11964635039100872708

碩士 === 慈濟大學 === 人類遺傳研究所 === 94 === Abstract Electroconvulsive shock (ECS) therapy is an induction of artificial seizures by passing electricity through the patient’s brain. Electroconvulsive shock therapy is effective in treating people who have major depression, mania, schizophrenia, or other psychiatric disorders. The mechanism of action underlying the effectiveness of electroconvulsive shock remains unknown. In this study, we aim to understand the mechanism of ECS by using two-dimensional gel electrophoresis (2-DE) and microarray analysis to identify differentially expressed genes in rat brains. We treated 10 male Sprague Dawley rats with repeated ECS, (45 mA, 2 s, once per day for 14 consecutive days), via ear-clip electrodes (UGO BASILE ECT UNIT 7801). Ten other male control rats were treated in the same way as the ECS-treated rats but with no electric current (sham treatment). In the 2-DE experiment, we identified several differentially expressed protein spots in the hippocampus of rats treated with ECS compared to control rats. These spots were analyzed by using mass spectrometry. seven identified proteins were quinoid dihydropteridine reductase, glutathione peroxidase (EC1.11.1.9) I pritein, similar to BC023835 protein, similar to mitochondrial ribosomal protein L15, chaperonin containing TCP1 subunit 2 (beta), Ubiquilin 1, and similar to neuron navigator. Expression of the glutathione peroxidase I was further confirmed by Western blot analysis. Unfortunately, the protein expression in ECS-treated and control rats was of no difference. As for the microarray experiment, we prepared total RNA from frontal cortex of rats; Microarray analysis was performed to detect differentially expressed genes. Furthermore, we used real-time quantitative PCR to confirm 10 out of 102 differentially expressed genes. Four genes showed significant difference between ECS-treated and control rats. They are Dbi, Mgst3, S100B, and S100A13. This study explored some novel genes that were regulated by repeated ECS treatment. To conclude, those ECS-induced genes may have the functions related to neuromodulatory, neuroprotection and metabolic detoxication.