USING QUARTZ CRYSTAL MICROBALANCE TO DEVELOP THE RAPID DETECTION BIOPLATFORM

碩士 === 大同大學 === 生物工程學系(所) === 94 === All of the bio-researcher would like to design the detection bioplatform that be more stable、sensitive、faster、convenient. The paper is based on using quartz crystal microbalance to research Vibrio parahamolyticus and breast cancer. The whole to say, the method of...

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Bibliographic Details
Main Authors: Hung-Che Chou, 周鴻哲
Other Authors: Tsong-Rong Yan
Format: Others
Language:en_US
Published: 2006
Online Access:http://ndltd.ncl.edu.tw/handle/04082715341135274689
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Summary:碩士 === 大同大學 === 生物工程學系(所) === 94 === All of the bio-researcher would like to design the detection bioplatform that be more stable、sensitive、faster、convenient. The paper is based on using quartz crystal microbalance to research Vibrio parahamolyticus and breast cancer. The whole to say, the method of Plasma Graft is the beat efficient to immobilize functionality on the golden surface. Then it will be more stability 10 times by using the method of PEI and GA chemistry immobili -zation way. As the GA’s move up, the DNA probe immobilization quantity lies to linear ascension. The ratio of DNA probe immobilization quantity is 2 percent on the chip, then the best hybridization immobilization quantity is up to 18 percent of the DNA probe immobilization quantity, and the reaction time only 10 minute. Another part of paper results is want to immobili -zation antibody and hybridization on the chip, the accidence test is feasible, and antibody still had activity to recognize specific antigen. The above-mentioned results to show the paper design’s bio-QCM platform can be accomplished accidence detectable result.