| Summary: | 碩士 === 國立陽明大學 === 微生物及免疫學研究所 === 94 === Kaposi’s sarcoma-associated herpesvirus (KSHV) is a human oncogenic virus first identified in HIV-infected patients, and is the causative agent of Kaposi’s sarcoma. K15 gene is located in the right most of the genome. There are two different forms of K15, predominant (K15P) and minor (K15M) form. Both K15P and K15M are twelve transmembrane proteins with a cytosolic carboxyl terminus and shared 33% amino acid identity. Predicted cytosolic carboxyl domain of K15P is similar to those found in EBV LMP1(Latent membrane protein 1). LMP1 can upregulate many genes, such as metalloproteinase 1 (MMP1), MMP2, MMP9. Overexpression of K15P enhanced cell invasion ability and upregulated angiogenesis-related genes expression, including MMP1 and urokinase plasmiongen activator (uPA). Overexpression of K15P in mouse fibroblast activatied AP-1 and enhanced invasion ability. Decreasing in AP-1 activity by using AP-1 inhibitor, NDGA, was correlated with a decreased invasion ability. K15P then promotes cell invasion ability via AP-1 activation.
|
|---|
