Study of the Behavior and Brain Region c-Fos Expression after Chronic Subanesthetic Dose of Ketamine Followed by 7 Days Withdrawal and a Further Challenge in Rats

• Main Authors: Shu-Wei Kuo, 郭書瑋
• Other Authors: Yn-Ho Huang
• Format: Others
• Language: zh-TW
• Published: 2006
• Online Access: http://ndltd.ncl.edu.tw/handle/31548371989517215423

碩士 === 國立陽明大學 === 解剖暨細胞生物學研究所 === 94 === Ketamine, which is non-competitive NMDA receptor antagonist, has been widely used as an intravenous or intramuscular anesthetic. Subanesthetic dose of ketamine produces several behavioral abnormalities in rodents which like the symptoms of schizophrenic patients. And it produces hyperlocomotion, stereotypy, cognitive impairments, and abnormal social interaction. In previous studies, which just focus on the acute effect of ketamine, and the studies about chronic subanesthetic dose of ketamine use after withdrawal produced behavior change and the ketamine-induced conditioned place preference is lack. In our study, we use conditioned place preference model to examine the ketamine dependence, and measure the locomotion activity, stereotypy, ataxia to check the behavior of rat, which after chronic subanesthetic dose of ketamine use. And use c-Fos IHC to identified the relationship between the behavior change and the brain region activation. We found that chronic subanesthetic ketamine(10mg/kg and 30mg/kg) use do not produce CPP in rat, after challenge dose inject, Ket/Ket group may induce behavior sensitization in rat, and after stop to use ketamine, the locomotion, stereotypy behavior and ataxia do not change. Chronic use of ketamine increase c-Fos expression in BLA, NAc; after challenge, the c-Fos increase in PrL, Cg, BLA, CA1 of hippocampus, but the meaning of those c-Fos expression is not clear. The TH positive cell number in VTA , SN do not change, it means that chronic ketamine(30mg/kg) use may not affect the function of dopaminergic neuron. In conclusion, chronic subanesthetic ketamine do not produce CPP ,and the behavior and dopaminergic neuron function do not change after stop to use ketamine.