Study on Ruthenium Oxide and Enzymatically Modified Electrode with Penicillin for Amperometric and Potentialmetric Measurements

• Main Authors: Sheng-Hung Chen, 陳省宏
• Other Authors: Jung-Chuan Chou
• Format: Others
• Language: zh-TW
• Published: 2006
• Online Access: http://ndltd.ncl.edu.tw/handle/98819789549072479150

碩士 === 國立雲林科技大學 === 電子與資訊工程研究所 === 94 === Along with the massive production, miniaturization, and integrations of the electrode or the signal conversion devices, the cost is obvious reducing, and the biosensor will be able to bring many conveniences for the daily life. Due to the penicillin drug-resistant tends to raise gradually, the accuracy of the use of medicine for the examination medical treatment raises immediately. Therefore, this thesis adopts the ruthenium oxide (RuOx) thin film as the substrate based on the penicillin enzymatically enzyme electrode, which has the characteristics of cheaper price and the simple manufactory. Nevertheless, it can be developed towards as the disposable type sensor, so it can have the time-limited efficiency of the clinical medical treatment immediately. In this thesis, R.F. sputtering technology is presented in fabricating thin film of ruthenium oxide based on extended gate field effect transistor (EGFET). This film structure can be simulated as sensing electrode of semiconductor process by means of I-V characteristic curve. In sputtering process, the preparation of the ruthenium oxide film is the main work for the optimization, which is deposited by R.F. sputtering in the low pressure. The sensing membrane of the ruthenium oxide presents a linear response from pH 1 to pH 13 of standard pH solutions with 54 mV/pH~ 57 mV/pH at the room temperature. Moreover, cyclic voltammetry (CV) is studied for the electrode of the basic characteristic and electrochemical behavior mechanism. Penicillin G Acylase (PGA) will be determined the penicillin G content, that possesses the single-minded property for the catalytic reaction, which shows the potentionmetric variation for penicillin G substance in the aqueous solution. Therefore, we immobilized PGA by employing the immobilized technique with 3-glycidoxypropyltrimethoxysilane (GPTS) in this study. The enzymatically modified electrode is placed in a 0.1 M phosphate buffer solution (PBS) pH 7.5 with several penicillin concentrations, the sensitivity of the penicillin enzymatically modified electrode is linear between 1 mM and 10 mM with a slope of 3.65 mV/mM. Sensitivity is a main factor for detecting membrane quality, as well as stability. In accordance with the above-mentioned, the RuOx bare electrode and enzymatically modified electrode are measured for drift effect and hysteresis effect, the characteristics of both electrodes can be obtained.