| Summary: | 碩士 === 中國醫藥大學 === 中國醫學研究所碩士班 === 95 === The aim of this study was to investigate that Chinese herbal medicine major compound and concentrations have good enhance for bone healing. And we combined with the biomaterial to the study on bone cell.
In this study, we used Liu’s stain, alkaline phosphates stain（ALP stain） and tart rate-resistant phosphates stain（TRAP stain）to observe the morphological change in the osteoblasts, the osteoclasts and the osteoblasts/ osteoclasts co-cultures after they were mixed with different concentrations of the herbal medicine. We used the MTT assay to observe the survival and proliferation activity of bone cells. The differentiation effect of the osteoblasts was evaluated with ALP activity and formation of mineralized nodules. In addition, the effect of herbal medicine on the differentiation of the osteoclasts was evaluated with TRAP activity. Finally, we used the best concentrations of the herbal medicine combined with the bone complex material to the study of bone tissue engineering in-vivo.
In this study, we found that both naringin and quercetin promote the proliferation and differentiation of the osteoblasts. They also promote the osteoclasts in more higher concentrations（10 mg/ml）. As for cinnamaldehyde, it was inhibited for both the osteoblasts and the osteoclasts. In addition, the GGT-TCM is a biodegradable material with good biocompatibility and osteoconduction.
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