| Summary: | 碩士 === 中國醫藥大學 === 藥物化學研究所碩士班 === 95 === Desmodium sequax WALL. and Phyllodium pulchellum L. belong to the family Leguminosae(Fabaceae). In past Phyllodium pulchellum was named Desmodium pulchellum. Desmodium sequax and Phyllodium pulchellum look similarly, so we need to find a method to identify them.
This research is mainly to discover the differences between Desmodium sequax and Phyllodium pulchellum by appearance (fruit, seed, pollen) and histological anatomy. And, we duplicate ITS (Internal Transcribed Spacer) region extracted from plant using by the technology of PCR (Polymerase chain reaction). ITS region is different in each species, even they are the same genus. So it is usually used to identify differences in plants. By sequencing, we found that ITS1 and ITS2 length of Desmodium sequax is 225 bp and 197 bp. ITS1 and ITS2 length of Phyllodium pulchellum is 225 bp and 209 bp.
We set up a quantitative method of HPLC to determine the content of rutin in Desmodium sequax and Phyllodium pulchellum. The result shows that the rutin content of Desmodium sequax is 0.004 % and of Phyllodium pulchellum is 0.026 %. This method has a high degree of sensitivity, reproducibility, precision and recovery rate. Base on HPLC method, we select 5 peak to set up fingerprint in order to identify Desmodium sequax and Phyllodium pulchellum.
In this study, we try to find a best method to identify Desmodium sequax and Phyllodium pulchellum. In future our aim is to find hepatoprotective effect in this two plants.
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