| Summary: | 碩士 === 嘉南藥理科技大學 === 化妝品科技研究所 === 95 === Dandruff is mainly due to speedy skin turnover which results in epidermal cells hyper proliferation and flaking off. When excessively sebum secrets, the number of Pityrosporum ovale, P. ovale, which resides in sebaceous glands, increases a lot. Dandruff can be controlled by antibiotics at present, but it may easily result in antibiotic drug resistance and skin inflammation.
The objective of this study is to investigate the feasibility of essential oils added in anti-dandruff products. In the first part of this study, the antimicrobial activity of P. ovale was determined by broth dilution method in terms of MIC (minimum inhibitory concentration). Acorus macrospadiceus demonstrated the best antimicrobial activity in the concentration of 0.055%, then was Cymbopogon citrates in 0.085%, and the remainders were between 0.1% and 0.2%.
The second part of this study was to verify the anti-inflammation ability of essential oils. Protein concentration of IL-8 in the supernatant of THP-1 cells was detected by ELISA. After LPS stimulating, Origanum x majoricum, Ocimum basilicum, Tagetes lucida and Agastache foeniculum had the ability to inhibit IL-8 secretion. Besides, after stimulated by 3 and 5 days’ P. ovale cultured supernatants, Agastache foeniculum also inhibited IL-8 secretion. This result demonstrated that Agastache foeniculum can inhibit inflammation in not only early but also late inflammatory stages.
Finally, the anti-P. ovale activity of products using essential oils were evaluated. Ten out of 12 essential oils showed inhibition ability higher than 95% after 72 hours inhibition period. The shampoo and scalp tonic containing Cymbopogon citrates had the inhibition ability of 100%.
In conclusion, essential oils had large potentials to be anti-dandruff shampoo additives.
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