Optimization of An Immunochromatographic Assembly and Rapid Detection to Streptavidin Protein

• Main Authors: Kai-Chih Wu, 吳凱智
• Other Authors: JUI-CHUANG WU
• Format: Others
• Language: zh-TW
• Published: 2007
• Online Access: http://ndltd.ncl.edu.tw/handle/89067117250642543750

碩士 === 中原大學 === 化學工程研究所 === 95 === Abstract: The immunochromatographic test （ICT）is one of the important biosensing techniques. The principle of ICT bases on the immunoaffinity partition of the analyte among antibodies and antigen. ICT has been widely used at clinical, custom house, and the public security bureau. In present research, the multi-analyte assay is qualitative or half- quantitative. In addition, it has many different types of chromophors as ICT visualization markers. In common usage, they are latex, colloid gold, enzyme, dye, and carbon black as mentioned in the literature. In this study, immunochromatographic test is adopted to detect the analyte (streptavidin), which migrates laterally through the porous NC membrane by the capillary force. The immunoreaction between mouse anti-streptavidin and biotinylated mouse anti-human IL6 is observed via the colloidal gold labeling. This research successfully assembed the test device and performed the detection of streptavidin. The detection limit was about 1pg/ml, same as the report in the literature of 10-12g/ml. [1]